[Expression of FAT1 in Lung Adenocarcinoma and Its Relationship 
with Immune Cell Infiltration].

BACKGROUND: Lung cancer is a leading cause of cancer-related deaths. Non-small cell lung cancer (NSCLC) is the most common pathological subtype, with adenocarcinoma being the predominant type. FAT atypical cadherin 1 (FAT1) is a receptor-like protein with a high frequency of mutations in lung adenocarcinoma. The protein encoded by FAT1 plays a crucial role in processes such as cell adhesion, proliferation, and differentiation. This study aims to investigate the expression of FAT1 in lung adenocarcinoma and its relationship with immune infiltration. METHODS: Gene expression levels and relevant clinical information of 513 lung adenocarcinoma samples and 397 adjacent lung samples were obtained through The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data. The mRNA expression levels of the FAT1 gene in lung adenocarcinoma tissues were analyzed, along with its association with the prognosis of lung adenocarcinoma patients. Pathway enrichment analysis was conducted to explore the signaling pathways regulated by the FAT1 gene. Immunoblotting was used to detect the differential expression of FAT1 in lung epithelial cells and various lung cancer cell lines, while immunohistochemistry was employed to assess FAT1 expression in lung cancer and adjacent tissues. RESULTS: FAT1 gene mutations were identified in 14% of lung adenocarcinoma patients. TCGA database data revealed significantly higher FAT1 mRNA expression in lung adenocarcinoma tissues compared to adjacent lung tissues. Kaplan-Meier analysis indicated lower survival rates in lung adenocarcinoma patients with higher FAT gene expression. Pathway enrichment analysis suggested the involvement of FAT1 in tumor development pathways, and its expression was closely associated with immune cell infiltration. Immunohistochemical validation demonstrated significantly higher expression of FAT1 in cancer tissues compared to adjacent lung tissues. CONCLUSIONS: FAT1 mRNA is highly expressed in lung adenocarcinoma tissues, and elevated FAT1 mRNA expression is associated with poor prognosis in lung adenocarcinoma patients. FAT1 may serve as a potential biomarker for lung cancer.

N-doped graphene encapsulated MoS2nanosphere composite as a high-performance anode for lithium-ion batteries.

MoS2is widely used in lithium-ion batteries (LIBs) due to its high capacity (670 mAh g-1) and unique two-dimensional structure. However, the further application was limited of MoS2as anode materials suffer from its volume expansion and low conductivity. In this work, N-doped graphene encapsulated MoS2nanosphere composite (MoS2@NG) were prepared and its unique sandwich structure containing abundant mesopores and defects can efficiently enhance reaction kinetics. The MoS2@NG electrode shows a reversible capacity of 975.9 mAh g-1at 0.1 A g-1after 100 cycles, and a reversible capacity of 325.2 mAh g-1is still maintained after 300 cycles at 5 A g-1. In addition, the MoS2@NG electrode exhibites an excellent rate performance benefiting from the electrochemical properties dominated by capacitive behavior. This suggests that MoS2@NG composite can be used as potential anode materials for LIBs.

Green fabrication of porous microspheres containing cellulose nanocrystal/MnO2 nanohybrid for efficient dye removal.

Microspheres based on cellulose nanocrystal (CNC)/metal oxide hybrid materials have great application prospects in wastewater treatment due to simultaneously adsorption, degradation ability, easily separation and recycling properties. However, the relatively small porosity and specific surface area of the CNC-based microspheres limit their adsorption ability. Herein, we reported a facile strategy to prepare porous microsphere based on CNC/MnO2 by freeze-drying the air-bubble templated emulsion, in which the sodium alginate (SA) was used as the crosslinked matrix. Thus-obtained CNC/MnO2/SA microspheres showed low density of 0.027 g/cm3 and high porosity of 98.23%. Benefiting from the high porosity, synergetic effect of CNC electrostatic adsorption and oxidative degradation ability of MnO2, the decolorization ratio of methylene blue (800 mg/mL) could be up to 95.4% in 10 min, and the equilibrium decolorization could reach 114.5 mg/g. This study provides a green and facile strategy to design porous CNC-based material for dye wastewater treatment.

Quantifiable stretching-induced fluorescence shifts of an elastically bendable and plastically twistable organic crystal.

Organic crystals with mechanical stimulus-response properties are being developed increasingly nowadays. However, the studies involving tensile-responsive crystals are still lacking due to the strict requirement of crystals with good flexibility. In this work, an organic crystal with the ability of elastic bending and plastic twisting upon loading stress and shearing force, respectively, is reported. The deformability in different directions enables the crystal to be a model for tensile-responsive study. Indeed, blue shifts of fluorescence were observed when the tensile forces loaded upon the needle-shaped crystal were stretched to a certain degree. The mathematical correlation between emission wavelength changes and stretching strain was obtained for the first time, which proves that the crystal has a potential application for tension sensors. In addition, a low detection limit and high sensitivity enabled the crystal to have the ability to detect tension variations in precision instruments. Theoretical calculations and X-ray crystal structure analyses revealed the mechanism of emission wavelength shifts caused by molecular movement during the stretching process. The presented crystal successfully overcame the limitations of traditional mechanochromic organic crystals, which have difficulty in responding to tensile forces.